Combined effect of bisphosphonate and recombinant human bone morphogenetic protein 2 on bone healing of rat calvarial defects
김호철, 송재민, 김창주, 윤상용, 김인령, 박봉수, 신상훈,
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김호철 ( Kim Ho-Chul ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
송재민 ( Song Jae-Min ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
김창주 ( Kim Chang-Joo ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
윤상용 ( Yoon Sang-Yong ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
김인령 ( Kim In-Ryoung ) - Pusan National University School of Dentistry Department of Oral Anatomy and Cell Biology
박봉수 ( Park Bong-Soo ) - Pusan National University School of Dentistry Department of Oral Anatomy and Cell Biology
신상훈 ( Shin Sang-Hun ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
Abstract
Background: This study aimed to investigate new bone formation using recombinant human bone morphogenetic protein 2 (rhBMP-2) and locally applied bisphosphonate in rat calvarial defects.
Methods: Thirty-six rats were studied. Two circular 5 mm diameter bony defect were formed in the calvaria using a trephine bur. The bony defect were grafted with Bio-Oss® only (group 1, n?=?9), Bio-Oss® wetted with rhBMP-2 (group 2, n?=?9), Bio-Oss® wetted with rhBMP-2 and 1 mM alendronate (group 3, n?=?9) and Bio-Oss® wetted with rhBMP-2 and 10 mM alendronate (group 4, n?=?9). In each group, three animals were euthanized at 2, 4 and 8 weeks after surgery, respectively. The specimens were then analyzed by histology, histomorphometry and immunohistochemistry analysis.
Results: There were significant decrease of bone formation area (p?
Conclusion: Low concentration bisphosphonate and rhBMP-2 have synergic effect on bone regeneration and this is result from the decreased activity of RANKL of osteoblast.
키워드
Bisphophonate; Bone morphogenetic protein 2; Bone regeneration; Histology; Alendronate
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